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产品描述：小鼠组蛋白去乙酰化酶复合物亚基SAP130(SAP130)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SAP130含量。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，本产品适用于基础科研领域，如探究表观遗传调控机制、建立炎症相关疾病动物模型、评估药物干预对SAP130表达的影响等研究场景，为探索SAP130在免疫调控和疾病发生中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sap130 ELISA Kit; Histone deacetylase complex subunit SAP130 ELISA Kit; 130 kDa Sin3-associated polypeptide ELISA Kit; Sin3-associated polypeptide p130 ELISA Kit
缩写：SAP130
Uniprot No.：Q8BIH0
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.72 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAP130 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of mouse SAP130 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 94 | 93-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.6222.5352.5792.43015002.1172.1542.1361.9877501.4021.5201.4611.3123750.7360.7800.7580.609187.50.4780.4950.4870.33893.750.3110.3160.3140.16546.880.2450.2400.2430.09400.1530.1440.149 | ||||||
货期：3-5 working days
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