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产品描述:人Salusin Beta(Salusin β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的Salusin β含量。试剂盒检测范围为0.45 pg/mL-30 pg/mL,适用于心血管疾病模型研究、代谢综合征相关分子机制探索、基础医学中生物活性肽的表达分析等科研领域,为揭示Salusin β在生理及病理状态下的功能提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Salusin Beta
缩写:Salusin β
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.45 pg/mL-30 pg/mL
灵敏度:0.11 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Salusin β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 83 | ||||
Range % | 80-86 | |||||
1:200 | Average % | 102 | ||||
Range % | 97-107 | |||||
1:400 | Average % | 110 | ||||
Range % | 107-113 | |||||
1:800 | Average % | 99 | ||||
Range % | 96-104 |
回收率:
The recovery of human Salusin β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 87 | 82-93 | ||||
EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected302.5982.5742.5862.491152.2012.2352.2182.1237.51.5961.5681.5821.4873.750.9030.9350.9190.8241.80.4660.4750.4710.3760.90.3270.3380.3330.2380.450.2250.2410.2330.13800.0940.0960.095 |
货期:3-5 working days
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