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产品描述：小鼠血清淀粉样蛋白A2(SAA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SAA2含量。SAA2即血清淀粉样蛋白A2，它与炎症反应密切相关。在机体受到感染、损伤等刺激时表达上调。研究机制方面，主要聚焦于其在炎症信号传导通路中的作用，以及对免疫细胞的调节，探索其作为炎症相关疾病诊断标志物和治疗靶点的潜力。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于基础科研中炎症机制研究、疾病模型动态监测或代谢相关实验的生物学标记分析。尤其适合体外实验如细胞因子诱导模型、感染性疾病动物模型的血清或组织样本检测，为炎症相关信号通路研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Saa2 ELISA Kit; Serum amyloid A-2 protein [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
缩写：SAA2
Uniprot No.：P05367
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SAA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 108 | |||||
| Range % | 98-112 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 88-101 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 83-94 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 90-104 | ||||||
回收率：
| The recovery of mouse SAA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 93-106 | |||||
| EDTA plasma (n=4) | 93 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected5001.7231.8351.7791.5912501.2771.1891.2331.0451250.8640.8870.8760.68862.50.6850.6740.6800.49231.20.4630.4560.4600.27215.60.3840.3670.3760.1887.80.2860.2910.2890.10100.1900.1860.188 | |||||||
货期：3-5 working days
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