首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血清淀粉样蛋白A(SAA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液样本中的SAA1含量。SAA1，即血清淀粉样蛋白A1，是一种急性期蛋白，主要由肝脏在炎症刺激下产生。SAA1在炎症反应中扮演重要角色，能够诱导Th17细胞分化，促进炎性疾病的发生。其表达受IL-1、IL-6和TNF-α等促炎细胞因子调控，并与HDL复合物中的载脂蛋白相互作用。试剂盒检测范围为156 ng/mL-10000 ng/mL，该产品可广泛应用于基础医学研究中SAA表达调控机制探索、炎症性疾病动物模型评估、生物标志物筛选验证等场景，支持科研人员通过血清或尿液样本进行SAA动态监测，为感染免疫研究、代谢性疾病分析及药物作用机制等课题提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SAA1 ELISA Kit; Serum amyloid A-1 protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA); Serum amyloid protein A(2-104); Serum amyloid protein A(3-104); Serum amyloid protein A(2-103); Serum amyloid protein A(2-102); Serum amyloid protein A(4-101)] ELISA Kit
缩写：SAA1
Uniprot No.：P0DJI8
种属：Homo sapiens (Human)
样本类型：serum, urine
检测范围：156 ng/mL-10000 ng/mL
灵敏度：39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 101 | ||||
| Range % | 90-110 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of human SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected100002.7212.5982.6602.54150002.1872.0872.1372.01825001.5421.4241.4831.36412500.9620.9410.9520.8336250.5790.5470.5630.444312.50.3870.3710.3790.2601560.2630.2590.2610.14200.1220.1160.119 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human SAA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled SAA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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