首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛血清淀粉样蛋白A(SAA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SAA1含量。SAA1即血清淀粉样蛋白 A1，它在机体天然免疫中发挥重要作用，炎症状态下其水平会显著升高。研究机制方面，它涉及多种细胞信号通路，与炎症反应、脂质代谢等密切相关，还可能是某些疾病的生物标志物或潜在治疗靶点。试剂盒检测范围为0.1 μg/mL-20 μg/mL，适用于畜牧兽医研究、动物疾病模型构建等领域，可用于探究炎症相关疾病的分子机制、评估疫苗或药物的免疫调节效果，以及监测饲养环境中牛群健康状态，为科研人员提供稳定可靠的实验数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SAA1 ELISA Kit; SAA ELISA Kit; Serum amyloid A protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
缩写：SAA1
Uniprot No.：P35541
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：0.1 μg/mL-20 μg/mL
灵敏度：0.05 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 90-95 | |||||
回收率：
| The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-99 | ||||
| EDTA plasma (n=4) | 96 | 89-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 01.9631.9771.970 0.11.6441.6541.649 0.41.2391.2451.242 1.60.8170.7980.808 50.3720.3680.370 200.1210.1230.122 | ||||||
货期：3-5 working days
×
