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产品描述:山羊血清淀粉样蛋白A(SAA)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测样本中的SAA含量。SAA 即血清淀粉样蛋白 A,是一种急性期蛋白。在炎症、感染等刺激下其水平会迅速升高。研究显示,SAA 可参与免疫调节、脂质代谢等过程,还可能与动脉粥样硬化、类风湿关节炎等疾病发生发展相关,在疾病诊断与病情监测上有潜在价值。支持科研人员高效开展山羊炎症性疾病机制研究、感染模型评估或养殖健康管理等相关实验,为动物免疫学研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:SAA
Uniprot No.:A5JST2
种属:Capra hircus (Goat)
样本类型:
检测范围:
灵敏度:
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of goat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 91-101 | |||||
1:2 | Average % | 92 | ||||
Range % | 87-96 | |||||
1:4 | Average % | 95 | ||||
Range % | 91-98 | |||||
1:8 | Average % | 96 | ||||
Range % | 91-103 |
回收率:
The recovery of goat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 92-105 | ||||
EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2Average 80000.1210.1260.124 30000.2080.2200.214 15000.3310.3540.343 5000.5940.5870.591 1001.2651.2491.257 02.6482.5442.596 |
货期:3-5 working days
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