首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪S100B蛋白(S-100B)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S-100B含量。S-100B是一种钙结合蛋白，主要存在于神经胶质细胞中。其背景上与神经系统损伤、疾病相关。研究机制方面，它可作为生物标志物反映中枢神经系统损伤程度，还参与细胞增殖、分化等过程，在神经病理生理活动中发挥重要作用。试剂盒检测范围为78 pg/mL-5000 pg/mL，本试剂盒通过高特异性抗体对目标蛋白进行捕获与检测，可稳定应用于神经损伤模型研究、疾病机制探索或药物干预效果评价等科研场景，为分析猪体内S100B蛋白的动态变化提供可靠工具。样本兼容性强，支持直接检测未经稀释的体液样本或经适当处理的组织匀浆，操作流程标准化，满足实验室对神经生物学、兽医学或比较医学等领域的研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：S-100B
Uniprot No.：I3L5R6
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 85-101 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 92-105 | |||||
回收率：
| The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 83-102 | ||||
| EDTA plasma (n=4) | 95 | 92-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.0252.0672.0461.88725001.5741.5241.5491.39012501.1831.1691.1761.0176250.8210.8120.8170.6583120.5670.5340.5510.3921560.3540.3730.3640.205780.2500.2620.2560.09700.1640.1530.159 | ||||||
货期：3-5 working days
×
