首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠S100-B蛋白(S100B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100B含量。S100B是一种钙结合蛋白，主要在神经胶质细胞中表达。其与神经系统疾病密切相关，如脑损伤、阿尔茨海默病等。研究机制上，它可作为生物标志物反映神经损伤程度，还能通过多种信号通路影响细胞生长、凋亡等过程以参与疾病发生发展。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本产品适用于神经退行性疾病模型（如阿尔茨海默病）、脑损伤机制研究或肿瘤微环境分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100bProtein S100-B ELISA kit; S-100 protein beta chain ELISA kit; S-100 protein subunit beta ELISA kit; S100 calcium-binding protein B ELISA kit
缩写：S100B
Uniprot No.：P50114
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 85-91 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of mouse S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 93-106 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5422.3662.4542.2681002.1532.1752.1641.978501.5461.5531.5501.364250.8210.8550.8380.65212.50.5250.5600.5430.3576.250.3290.3250.3270.1413.120.2660.2560.2610.07500.1880.1840.186 | ||||||
货期：3-5 working days
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