首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：犬S100B蛋白(S100B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100B含量。S100B是一种钙结合蛋白，主要存在于神经胶质细胞中。在神经系统疾病如阿尔茨海默病、脑损伤中，S100B水平会出现异常。研究发现它可通过多种信号通路影响神经细胞生长、凋亡等，其异常表达可能参与疾病的发生发展。试剂盒检测范围为39 pg/mL-2500 pg/mL，该产品适用于神经科学基础研究、疾病模型评估及药物开发等科研场景，例如通过检测外周血或脑组织样本中的S100B水平，探究犬类神经退行性病变机制或创伤性脑损伤的生物标志物动态变化，为动物实验提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：S100B
Uniprot No.：E2QTP3
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 80-91 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 87-98 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-106 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
回收率：
| The recovery of pig S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-102 | |||||
| EDTA plasma (n=4) | 89 | 84-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 25001.9552.0211.9881.816 12501.4741.5221.4981.326 6250.9110.9870.9490.777 312.50.5260.5360.5310.359 156.20.3650.3450.3550.183 78.10.2930.3120.3030.131 390.2210.2260.2240.052 00.1750.1690.172 | |||||||
货期：3-5 working days
×
