首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠S100-A9蛋白(S100A9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的S100A9含量。S100A9是一种钙离子结合蛋白。它在炎症反应、免疫调节等生理和病理过程中发挥作用，其表达异常与多种疾病如肿瘤、自身免疫病等密切相关。研究机制聚焦于其在细胞信号传导、细胞迁移等方面的作用以及与相关疾病发生发展的关联。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于小鼠模型中的炎症机制研究、免疫细胞功能分析以及相关疾病的分子机制探索。产品包含标准品、检测抗体、酶结合物、预混显色液等核心组分及详细操作说明书，适用于实验室常规条件下开展基础研究与药物开发相关的蛋白表达水平检测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：S100a9 ELISA Kit; Cagb ELISA Kit; Mrp14 ELISA Kit; Protein S100-A9 ELISA Kit; Calgranulin-B ELISA Kit; Leukocyte L1 complex heavy chain ELISA Kit; Migration inhibitory factor-related protein 14 ELISA Kit; MRP-14 ELISA Kit; p14 ELISA Kit; S100 calcium-binding protein A9 ELISA Kit
缩写：S100A9
Uniprot No.：P31725
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-92 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of mouse S100A9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-99 | ||||
| EDTA plasma (n=4) | 96 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.8232.9882.9062.747152.1202.2302.1752.0167.51.5091.5041.5071.3483.750.8420.8910.8670.7081.80.5180.5550.5370.3780.90.3860.3820.3840.2250.450.2600.2540.2570.09800.1570.1600.159 | ||||||
货期：3-5 working days
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