关键信息
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基因名
Osteopontin/OPN
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简介
Osteopontin/OPN Protein, a key non-collagenous bone protein, strongly binds to hydroxyapatite, crucial for the mineralized matrix.It likely plays a vital role in mediating cell-extracellular matrix interactions.Additionally, as a cytokine, OPN stimulates interferon-gamma and interleukin-12 production, while inhibiting interleukin-10, pivotal for the type I immunity pathway.Osteopontin/OPN Protein, Rat (CHO, His) is the recombinant rat-derived Osteopontin/OPN protein, expressed by CHO , with C-6*His labeled tag.
- 应用
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生物活性
Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 10^5 cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37°C. The ED50 for this effect is < 1.50 µg/mL. Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x 105 cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37°C. The ED50 for this effect is 1.281 μg/ml, corresponding to a specific activity is 780.640 units/mg.
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别名
Osteopontin; Spp1; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1; 2b7; Secreted Phosphoprotein 1 [BNSP]
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种属
Rat
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表达系统
CHO
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P08721
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表达区间
L17-N317
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氨基酸序列
LPVKVAEFGSSEEKAHYSKHSDAVATWLKPDPSQKQNLLAPQNSVSSEETDDFKQETLPSNSNESHDHMDDDDDDDDDGDHAESEDSVNSDESDESHHSDESDESFTASTQADVLTPIAPTVDVPDGRGDSLAYGLRSKSRSFPVSDEQYPDATDEDLTSRMKSQESDEAIKVIPVAQRLSVPSDQDSNGKTSHESSQLDEPSVETHSLEQSKEYKQRASHESTEQSDAIDSAEKPDAIDSAERSDAIDSQASSKASLEHQSHEFHSHEDKLVLDPKSKEDDRYLKFRISHELESSSSEVN
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蛋白长度
Full Length of Mature Protein
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分子量
Predict MW: 34.83 kDa; 60-70 kDa band in SDS-PAGE under reducing conditions
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Osteopontin (OPN) is a glycoprotein that plays a crucial role in various physiological and pathological processes, including bone remodeling, immune response, and cellular adhesion. Initially identified as a component of the bone matrix, OPN has since been implicated in a range of diseases, including cancer, cardiovascular disorders, and chronic inflammatory conditions. The ability of OPN to interact with various cell types through its multiple binding sites facilitates its involvement in cellular signaling pathways, making it a valuable target for therapeutic intervention. Recent research has focused on the production and characterization of recombinant OPN proteins to better understand its biological functions and mechanisms of action. Recombinant OPN proteins enable researchers to elucidate the specific roles of OPN in cell adhesion, migration, and tissue remodeling, providing insights into its potential as a biomarker for disease progression and as a therapeutic target. Furthermore, the study of OPN's interactions with other proteins and its post-translational modifications is essential for developing novel strategies to modulate its activity in various clinical contexts. As such, ongoing investigations into the structure, function, and applications of recombinant osteopontin proteins continue to shed light on their significance in health and disease.
