首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人S100钙结合蛋白P(S100P)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的S100P含量。S100P是一种钙结合蛋白。它在多种肿瘤组织中呈现高表达，参与细胞增殖、分化和凋亡等多个生理过程。研究发现其可通过与受体结合激活下游信号通路，影响肿瘤细胞生长、迁移和侵袭，有望成为肿瘤诊断和治疗的潜在靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于体外实验中对S100P表达水平的动态监测，例如疾病机制研究、药物开发中生物标志物分析或肿瘤相关分子通路探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MIG9 ELISA Kit; Migration inducing gene 9 ELISA Kit; Protein S100-E ELISA Kit; Protein S100-P ELISA Kit; Protein S100P ELISA Kit; S100 calcium binding protein P ELISA Kit; S100 calcium-binding protein P ELISA Kit; S100 P ELISA Kit; S100E ELISA Kit; S100P ELISA Kit; S100P_HUMAN ELISA Kit
缩写：S100P
Uniprot No.：P25815
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human S100P in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:10 | Average % | 89 | ||||
| Range % | 82-93 | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of human S100P spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.6842.9012.7932.63125001.9631.9991.9811.81912501.1681.1211.1450.9836250.6320.6520.6420.480312.50.4150.4360.4260.2641560.3090.3200.3150.153780.2680.2550.2620.10000.1610.1630.162 | ||||||
货期：3-5 working days
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