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产品描述：猪血清淀粉样蛋白A(SAA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的SAA含量。试剂盒检测范围为19.53 pg/mL-5000 pg/mL，适用于科研领域中对猪免疫应答机制、感染性疾病模型或疫苗效果评估等研究。通过精确检测SAA水平，研究者可动态监测实验动物炎症反应进程，为畜牧养殖健康管理、病理机制探索及药物干预效果分析提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SAA2; Serum amyloid A-2 protein
缩写：SAA
Uniprot No.：P0DSO0
种属：Sus scrofa (Pig)
样本类型：serum, plasma
检测范围：19.53 pg/mL-5000 pg/mL
灵敏度：19.53 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:2000 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4000 | Average % | 101 | ||||
| Range % | 98-105 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 86-96 | |||||
回收率：
| The recovery of pig SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 88-99 | ||||
| EDTA plasma (n=4) | 103 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 50000.2040.2060.205 12500.4630.4710.467 312.50.9150.9350.925 78.11.4291.4621.446 19.532.0562.1382.097 02.5152.6872.601 | ||||||
货期：3-5 working days
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