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产品描述：绵羊血清淀粉样蛋白A(SAA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SAA1含量。试剂盒检测范围为1.875 ng/mL-120 ng/mL，特别适用于畜牧科研领域，可用于绵羊炎症性疾病模型建立、疫苗免疫效果评价、抗炎药物筛选等基础研究，亦能为动物病理学研究中组织炎症程度的定量评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SAA1 ELISA Kit; Serum amyloid A protein ELISA Kit; SAA ELISA Kit
缩写：SAA1
Uniprot No.：P42819
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：1.875 ng/mL-120 ng/mL
灵敏度：0.47 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 98-108 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 84-93 | |||||
回收率：
| The recovery of sheep SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-91 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average1200.0860.0880.087600.1360.1420.139300.2470.2310.239150.3620.3720.3677.50.5530.5660.5603.750.9240.9840.9541.8751.3241.4011.36302.4482.5432.496 | ||||||
货期：3-5 working days
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