首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Sal样蛋白4(SALL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SALL4含量。SALL4 作为重要靶点，其背景上在胚胎发育中起关键作用，异常表达与多种癌症发生发展相关。研究机制方面，主要聚焦其调控下游基因表达的分子机制，以及在肿瘤干细胞自我更新、肿瘤转移和耐药中的作用，为癌症靶向治疗提供新思路。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，该产品适用于体外实验研究，可用于探索SALL4在干细胞生物学、肿瘤微环境调控或疾病分子机制中的功能，如通过检测细胞模型、动物组织或临床前样本中的蛋白表达动态，为相关领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AA407717 ELISA Kit; AL022809 ELISA Kit; AW536104 ELISA Kit; C330011P20Rik ELISA Kit; C78083 ELISA Kit; C78563 ELISA Kit; dJ1112F19.1 ELISA Kit; DRRS ELISA Kit; HSAL4 ELISA Kit; Sal like 4 (Drosophila) ELISA Kit; Sal like 4 ELISA Kit; Sal like Protein 4 ELISA Kit; Sal-like protein 4 ELISA Kit; Sall4 ELISA Kit; SALL4_HUMAN ELISA Kit; Spalt like transcription factor 4 ELISA Kit; Tex20 ELISA Kit; Zinc finger protein 797 ELISA Kit; Zinc finger protein SALL4 ELISA Kit; ZNF797 ELISA Kit
缩写：SALL4
Uniprot No.：Q9UJQ4
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：37.5 pg/mL-2400 pg/mL
灵敏度：9.37 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SALL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human SALL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 101-107 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected24002.7822.7992.7912.65812001.9211.9651.9431.8106001.0821.0981.0900.9573000.5400.5640.5520.4191500.3350.3370.3360.203750.2430.2510.2470.11437.50.1900.1780.1840.05100.1320.1340.133 | ||||||
货期：3-5 working days
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