首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人可溶性CD146分子(sCD146)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的sCD146含量。sCD146 是一个值得关注的靶点。它是膜蛋白 CD146 的可溶形式。在肿瘤、心血管疾病等领域研究较多，其机制可能涉及参与细胞黏附、迁移等过程，影响肿瘤细胞侵袭转移、血管生成等，为疾病诊断与治疗提供新思路。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于体外研究sCD146在心血管疾病、肿瘤微环境或免疫调节中的表达水平变化，为探索其分子机制提供定量分析工具。支持科研人员在细胞生物学、病理模型或生物标志物筛选等非临床领域开展实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A32 antigen ELISA Kit; CD 146 ELISA Kit; CD146 ELISA Kit; CD146 antigen ELISA Kit; Cell surface glycoprotein MUC18 ELISA Kit; Cell surface glycoprotein P1H12 ELISA Kit; Gicerin ELISA Kit; Mcam ELISA Kit; Melanoma adhesion molecule ELISA Kit; Melanoma associated antigen A32 ELISA Kit; Melanoma associated antigen MUC18 ELISA Kit; Melanoma associated glycoprotein MUC18 ELISA Kit; Melanoma cell adhesion molecule ELISA Kit; Melanoma-associated antigen A32 ELISA Kit; Melanoma-associated antigen MUC18 ELISA Kit; MelCAM ELISA Kit; MUC 18 ELISA Kit; MUC18 ELISA Kit; MUC18_HUMAN ELISA Kit; S endo 1 ELISA Kit; S endo 1 endothelial associated antigen ELISA Kit; S-endo 1 endothelial-associated antigen ELISA Kit
缩写：sCD146
Uniprot No.：P43121
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.31 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD146 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:10 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:20 | Average % | 87 | ||||
| Range % | 83-90 | |||||
| 1:40 | Average % | 104 | ||||
| Range % | 101-104 | |||||
回收率：
| The recovery of human sCD146 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.9522.9682.9602.777402.3832.3752.3792.196201.6151.6461.6311.448100.9720.9990.9860.80350.5950.5780.5870.4042.50.4370.4680.4530.2701.250.3640.3570.3610.17800.1820.1840.183 | ||||||
货期：3-5 working days
×
