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产品描述：人分泌球蛋白家族1D成员2(SCGB1D2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SCGB1D2含量。SCGB1D2是一种分泌珠蛋白，主要在汗腺细胞产生，具有潜在的抗莱姆病作用。研究发现，SCGB1D2能显著抑制伯氏疏螺旋体生长，但其变异形式效果较差，可能与氨基酸序列变化有关。研究旨在利用SCGB1D2的抗菌特性，开发新型预防和治疗莱姆病的方法。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，适用于基础科研中探索SCGB1D2在生殖生物学、炎症性疾病或肿瘤微环境中的分子机制，也可用于评估动物模型或细胞实验中该蛋白的表达动态变化，支持研究者高效开展SCGB1D2相关的蛋白质组学或分子病理学研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SCGB1D2 ELISA kit; LIPHB ELISA kit; LPNBSecretoglobin family 1D member 2 ELISA kit; Lipophilin-B ELISA kit
缩写：SCGB1D2
Uniprot No.：O95969
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB1D2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 84-102 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:40 | Average % | 103 | ||||
| Range % | 99-110 | |||||
回收率：
| The recovery of human SCGB1D2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 80-90 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20002.4362.3512.3942.23310001.8721.8071.8401.6795001.1541.1821.1681.0072500.7950.8340.8150.6541250.4280.4480.4380.27762.50.3620.3410.3520.19131.250.2310.2170.2240.06300.1630.1590.161 | ||||||
货期：3-5 working days
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