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产品描述:人分泌球蛋白3A成员2(SCGB3A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SCGB3A2含量。SCGB3A2是重要靶点。它是分泌蛋白,在呼吸道上皮细胞表达。研究发现其与肺部疾病如肺纤维化、哮喘等密切相关。机制上,它能调节炎症反应、维持上皮细胞稳态,针对其开发的药物可干预相关疾病进程,有潜在治疗价值。试剂盒检测范围为46.88 pg/mL-3000 pg/mL,本产品适用于基础医学研究中SCGB3A2蛋白表达动力学分析,如在呼吸道疾病模型中的功能探究、免疫调节机制研究或作为潜在生物标志物的筛选验证,为探索其在肺部炎症、过敏性疾病或肿瘤微环境中的作用提供可靠检测工具,所有应用场景仅限于科研用途。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:LU103 ELISA Kit; Pneumo secretory protein 1 ELISA Kit; PnSP-1 ELISA Kit; PNSP1 ELISA Kit; SCGB3A2 ELISA Kit; Secretoglobin family 3A member 2 ELISA Kit; SG3A2_HUMAN ELISA Kit; UGRP1 ELISA Kit; Uteroglobin-related protein 1 ELISA Kit
缩写:SCGB3A2
Uniprot No.:Q96PL1
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:46.88 pg/mL-3000 pg/mL
灵敏度:11.72 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Epigenetics and Nuclear Signaling
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 95 | ||||
Range % | 91-98 | |||||
1:2 | Average % | 88 | ||||
Range % | 81-92 | |||||
1:4 | Average % | 104 | ||||
Range % | 98-111 | |||||
1:8 | Average % | 97 | ||||
Range % | 83-93 |
回收率:
The recovery of human SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 91 | 85-99 | ||||
EDTA plasma (n=4) | 95 | 89-100 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2AverageCorrected30002.7032.7522.7282.60615001.9061.8661.8861.7647501.1281.2001.1641.0423750.5450.5390.5420.420187.50.3160.3320.3240.20293.750.2050.2080.2070.08546.880.1860.2000.1930.07100.1210.1220.122 |
货期:3-5 working days
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