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产品描述：人乳腺珠蛋白-B(SCGB2A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SCGB2A1含量。试剂盒检测范围为0.235 ng/mL-15 ng/mL，适用于基础科研中探索SCGB2A1在乳腺生理功能、肿瘤微环境互作或病理进程中的生物学作用，例如通过检测不同处理条件下细胞模型、动物模型或临床样本中SCGB2A1的动态变化，解析其在细胞增殖、分化或信号通路中的分子机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Lacryglobin ELISA Kit; LIPHC ELISA Kit; Lipophilin C ELISA Kit; Lipophilin-C ELISA Kit; LPHC ELISA Kit; LPNC ELISA Kit; Mammaglobin 2 ELISA Kit; Mammaglobin-2 ELISA Kit; Mammaglobin-B ELISA Kit; MGB2 ELISA Kit; MGC71973 ELISA Kit; Scgb2a1 ELISA Kit; Secretoglobin family 2A member 1 ELISA Kit; Secretoglobin; family 2A; member 1 ELISA Kit; SG2A1_HUMAN ELISA Kit; UGB3 ELISA Kit
缩写：SCGB2A1
Uniprot No.：O75556
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.235 ng/mL-15 ng/mL
灵敏度：0.058 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB2A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-89 | |||||
回收率：
| The recovery of human SCGB2A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 92 | 88-87 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected152.3452.2522.2992.1587.51.7231.6761.7001.5593.751.2151.1541.1851.0441.880.7210.7430.7320.5910.940.4360.4410.4390.2980.470.3020.2880.2950.1540.2350.2340.2190.2270.08600.1430.1390.141 | ||||||
货期：3-5 working days
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