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产品描述：小鼠分泌球蛋白家族3A成员2(SCGB3A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SCGB3A2含量。SCGB3A2（分泌性杯状细胞相关蛋白 3A2）是重要的肺上皮细胞分泌蛋白。它常作为肺脏相关疾病的潜在靶点。研究机制方面，主要聚焦其在肺组织稳态维持、炎症反应调控等过程中的作用，探索通过干预它来治疗如肺纤维化等疾病。试剂盒检测范围为9.4 pg/mL-600 pg/mL，适用于小鼠模型中肺纤维化、哮喘等呼吸道疾病的分子机制探索、药物干预效果评估及SCGB3A2表达调控研究，支持科研人员高效开展体外实验。本产品仅限用于科学研究领域，不适用于其他用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Scgb3a2 ELISA Kit; Pnsp1 ELISA Kit; Ugrp1 ELISA Kit; Secretoglobin family 3A member 2 ELISA Kit; Pneumo secretory protein 1 ELISA Kit; PnSP-1 ELISA Kit; Uteroglobin-related protein 1 ELISA Kit
缩写：SCGB3A2
Uniprot No.：Q920H1
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：9.4 pg/mL-600 pg/mL
灵敏度：2.35 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 110 | |||||
| Range % | 104-114 | ||||||
回收率：
| The recovery of mouse SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected6002.5112.4682.4902.3183001.6721.6991.6861.5141501.0150.9891.0020.830750.5940.5860.5900.41837.50.4030.4120.4080.23618.750.2920.2890.2910.1199.40.2180.2070.2130.04100.1710.1730.172 | |||||||
货期：3-5 working days
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