首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质细胞衍生因子1a(SDF-1a/CXCL12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SDF1a/CXCL12含量。SDF1a/CXCL12是重要的趋化因子。其背景上，在胚胎发育、免疫调节等生理过程发挥关键作用。研究机制方面，与受体CXCR4结合，介导细胞迁移、增殖和存活，在肿瘤转移、炎症等病理进程中有重要影响，是相关疾病治疗潜在靶点。试剂盒检测范围为0.78 pg/mL-50 pg/mL，可广泛应用于神经再生、造血调控、免疫细胞趋化性等基础研究领域，为探索SDF - 1a/CXCL12在疾病模型中的动态变化提供高特异性检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SDF1a/CXCL12
Uniprot No.：Q9QZD1
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 pg/mL-50 pg/mL
灵敏度：0.195 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 83-97 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:400 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 81-95 | |||||
回收率：
| The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-96 | ||||
| EDTA plasma (n=4) | 97 | 94-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected502.8302.7752.8032.666252.3262.4532.3902.25312.51.6001.6321.6161.4796.250.9370.8940.9160.7793.120.5280.5320.5300.3931.560.3210.3350.3280.1910.780.1570.1480.1530.01600.1400.1330.137 | ||||||
货期：3-5 working days
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