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产品描述：人臂板蛋白3A(Sema 3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SEMA3A含量。SEMA3A是一个值得关注的靶点。它参与神经发育、血管生成等生理过程。在研究机制上，其与受体结合后通过信号通路影响细胞行为。在肿瘤、神经系统疾病等领域的研究中发现其作用与疾病发展相关，为治疗干预提供新思路。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外细胞模型、动物组织或体液样本中Sema 3A的定量检测，可支持神经生物学、肿瘤微环境调控或免疫信号通路等基础研究领域，为探索Sema 3A在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SEMA3A ELISA Kit; SEMAD ELISA Kit; Semaphorin-3A ELISA Kit; Semaphorin III ELISA Kit; Sema III ELISA Kit
缩写：SEMA3A
Uniprot No.：Q14563
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Sema 3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 96 | |||||||||||
| Range % | 85-100 | ||||||||||||
| 1:2 | Average % | 87 | |||||||||||
| Range % | 82-93 | ||||||||||||
| 1:4 | Average % | 98 | |||||||||||
| Range % | 95-100 | ||||||||||||
| 1:8 | Average % | 92 | |||||||||||
| Range % | 88-94 | ||||||||||||
回收率：
| The recovery of human Sema 3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 92 | 88-96 | |||||||||||
| EDTA plasma (n=4) | 97 | 90-104 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected102.4902.5692.5302.42252.1712.1962.1842.0762.51.7261.7561.7411.6331.251.2841.3061.2951.1870.6250.7270.7900.7590.6510.3120.4610.4810.4710.3630.1560.2870.2960.2920.18400.1070.1090.108 | |||||||||||||
货期：3-5 working days
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