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Rat P-Selectin ELISA kit

  • 中文名： 大鼠P选择素(P-Selectin/CD62P)ELISA kit
货号: IPD-E07399r
Price： ￥2500
数量：
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产品详情

  • 产品描述：大鼠P选择素(P-Selectin/CD62P)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELP含量。SELP即选择素P，主要表达于活化的血小板和内皮细胞。它参与炎症反应、血栓形成等病理生理过程。研究机制方面，SELP通过与配体结合，介导白细胞、血小板与内皮细胞的黏附，在肿瘤转移、动脉粥样硬化等疾病机制中扮演重要角色。试剂盒检测范围为9.38 ng/mL-600 ng/mL，灵敏度为2.34 ng/mL。在科研领域，广泛用于心血管疾病模型、炎症反应机制、血栓性疾病研究及药物干预效果评估等方向，例如通过检测动物模型血液或组织中的P选择素水平，揭示其在血管内皮损伤、血小板活化等生理病理过程中的动态变化，为相关基础研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P

  • 缩写：SELP

  • Uniprot No.：P98106

  • 种属：Rattus norvegicus (Rat)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：9.38 ng/mL-600 ng/mL

  • 灵敏度：2.34 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Cardiovascular

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %93
    Range %85-99
    1:2Average %92
    Range %84-105
    1:4Average %97
    Range %92-103
    1:8Average %94
    Range %82-98

  • 回收率：

    The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9795-100
    EDTA plasma (n=4)9592-100














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected6002.7552.8122.7842.6163002.2172.2362.2272.0591501.5451.5241.5351.367751.0510.9791.0150.84737.50.5870.6220.6050.43718.750.4560.4380.4470.2799.380.2880.2790.2840.11600.1730.1620.168　






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