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产品描述：人硒结合蛋白1 (SELENBP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SELENBP1含量。人硒结合蛋白1（SELENBP1）是一种与硒结合的蛋白，其在多种癌症类型中可能发挥抗癌作用。其表达水平与免疫浸润和免疫治疗密切相关，研究显示SELENBP1在结直肠癌中表达降低，与不良预后相关，且其表达影响肿瘤免疫浸润和化疗、免疫治疗的敏感性。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于体外研究SELENBP1在疾病模型、药物干预实验或生理条件下的表达调控，例如通过分析肿瘤组织匀浆或炎症动物模型的血清样本，探究其与氧化应激相关通路的关联性。科研人员可利用定量评估不同处理条件下SELENBP1的动态变化，为分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：56 kDa selenium binding protein ELISA Kit; 56 kDa selenium-binding protein ELISA Kit; hSBP ELISA Kit; LPSB ELISA Kit; SBP ELISA Kit; SBP1 ELISA Kit; SBP1_HUMAN ELISA Kit; SBP56 ELISA Kit; Selenbp1 ELISA Kit; Selenbp2 ELISA Kit; Selenium Binding Protein 1 ELISA Kit; Selenium binding protein 2 ELISA Kit; Selenium binding protein1 ELISA Kit; Selenium-binding protein 1 ELISA Kit; SELNBP1 ELISA Kit; SP56 ELISA Kit
缩写：SELENBP1
Uniprot No.：Q13228
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SELENBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SELENBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.5202.6042.5622.36740002.2032.2142.2092.01420001.8881.8991.8941.69910001.4451.4961.4711.2765001.0051.0671.0360.8412500.6730.6810.6770.4821250.3780.3840.3810.18600.1940.1960.195 | ||||||
货期：3-5 working days
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