首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人可溶性弹性蛋白片段(sELAF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的sELAF含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研中探究弹性蛋白代谢异常相关疾病模型、药物干预效果评估或生物标记物筛选，例如通过分析心血管疾病患者血清或肺纤维化动物模型组织匀浆中的sELAF水平，揭示其与疾病进展的关联性。支持科研人员高效开展弹性蛋白代谢调控机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sELAF
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sELAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of human sELAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 91-102 | ||||
| EDTA plasma (n=4) | 87 | 82-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.8212.7772.7992.6761001.8861.8261.8561.733501.2361.2221.2291.106250.7190.7390.7290.60612.50.3830.3740.3790.2566.250.2760.2840.2800.1573.120.1900.1850.1880.06500.1210.1250.123 | ||||||
货期：3-5 working days
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