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Mouse Semaphorin-3A(SEMA3A) ELISA kit

  • 中文名： 小鼠脑信号蛋白3A(SEMA3A)ELISA kit
货号: IPD-EL020980MO
Price： ￥2500
数量：
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产品详情

  • 产品描述：小鼠脑信号蛋白3A(SEMA3A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SEMA3A含量。SEMA3A属于分泌型蛋白，在神经系统发育中发挥导向作用。研究发现其与肿瘤、炎症等疾病相关。机制上，SEMA3A可与受体结合，调控细胞信号通路，影响细胞迁移、增殖和血管生成等过程，是潜在的药物作用靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品为神经科学、肿瘤生物学等领域的基础研究提供工具，支持细胞模型、动物模型中SEMA3A功能探索及信号通路研究，适用于体外实验的蛋白表达检测与调控机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Sema3a ELISA Kit; Semad ELISA Kit; SemD ELISA Kit; Semaphorin-3A ELISA Kit; Semaphorin III ELISA Kit; Sema III ELISA Kit; Semaphorin-D ELISA Kit; Sema D ELISA Kit

  • 缩写：SEMA3A

  • Uniprot No.：O08665

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma, cell culture supernates, tissue homogenates

  • 检测范围：15.6 pg/mL-1000 pg/mL

  • 灵敏度：3.9 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Cardiovascular

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:100Average %88
    Range %84-92
    1:200Average %94
    Range %90-98
    1:400Average %99
    Range %95-104
    1:800Average %102
    Range %96-108

  • 回收率：

    The recovery of mouse SEMA3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)8993-96
    EDTA plasma (n=4)10095-105














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected 10002.5102.5682.5392.392 5002.3612.2122.2872.140 2501.8691.8481.8591.712 1251.2471.2261.2371.090 62.50.6480.6690.6590.512 31.20.3710.3870.3790.232 15.60.2570.2610.2590.112 00.1460.1480.147　 






  • 货期：3-5 working days

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