首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠臂板蛋白-3F(SEMA3F)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SEMA3F含量。SEMA3F是一种在细胞运动和粘附中发挥作用的蛋白，参与细胞动力学和神经系统发育。其研究背景涉及其在细胞运动和粘附中的调节机制，以及作为候选抑癌基因在肿瘤中的作用。研究机制主要围绕其与受体结合，调节瘤细胞和内皮细胞功能，影响肿瘤生长和脉管生成。试剂盒检测范围为0.312 ng/mL-20 ng/mL，支持跨物种交叉反应验证，适用于探索SEMA3F在神经系统疾病模型、肿瘤侵袭性研究或炎症相关实验中的动态表达特征本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Sema3f ELISA Kit; Semaphorin-3F ELISA Kit; Semaphorin IV ELISA Kit; Sema IV ELISA Kit
缩写：SEMA3F
Uniprot No.：O88632
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SEMA3F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-103 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 87-97 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-106 | ||||||
回收率：
| The recovery of mouse SEMA3F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 88-94 | |||||
| EDTA plasma (n=4) | 107 | 103-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.5672.6992.6332.529102.1442.2442.1942.09051.5691.6251.5971.4932.51.0250.9891.0070.9031.250.7480.7460.7470.6430.6250.4620.4540.4580.3540.3120.2100.2230.2170.11300.1030.1050.104 | |||||||
货期：3-5 working days
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