首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪纤溶酶原激活物抑制因子1(PAI-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINE1含量。SERPINE1 即纤溶酶原激活物抑制剂 -1，它在多种生理和病理过程中起关键作用。其背景与凝血、纤维蛋白溶解等相关。研究机制方面，它可抑制纤溶酶原激活物，调节细胞迁移、增殖等，在肿瘤、心血管疾病等发生发展中有重要影响。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于心血管疾病模型、代谢综合征研究、创伤修复机制探索等科研领域，为分析PAI-1在凝血 - 纤溶系统失衡、器官纤维化或肿瘤微环境中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SERPINE1 ELISA Kit; PAI1 ELISA Kit; PLANH1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写：SERPINE1
Uniprot No.：P79335
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-93 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of pig PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-97 | ||||
| EDTA plasma (n=4) | 98 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.0502.0122.0311.905101.6691.6761.6731.54751.3971.3661.3821.2562.50.9960.9870.9920.8661.250.6680.6780.6730.5470.6250.4640.4570.4610.3350.3120.3030.2990.3010.17500.1250.1270.126 | ||||||
货期：3-5 working days
×
