首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胶质细胞源性连接蛋白(SERPINE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINE2含量。SERPINE2是重要靶点。它在细胞生长、迁移、黏附及组织重塑等生理过程有作用。研究机制方面，涉及调节蛋白酶活性等，其异常表达与肿瘤、神经系统疾病等病理状况相关，深入研究能为相关疾病治疗提供新方向。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品可广泛应用于神经退行性疾病模型（如阿尔茨海默病）、中枢神经损伤修复机制、胶质瘤微环境研究等科研领域，特别适用于探究脑卒中、脊髓损伤等病理过程中胶质细胞功能动态变化，以及肿瘤微血管生成相关分子机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Serpine2 ELISA Kit; Pi7 ELISA Kit; Pn1 ELISA Kit; Spi4Glia-derived nexin ELISA Kit; GDN ELISA Kit; Peptidase inhibitor 7 ELISA Kit; PI-7 ELISA Kit; Protease nexin 1 ELISA Kit; PN-1 ELISA Kit; Protease nexin I ELISA Kit; Serine protease-inhibitor 4 ELISA Kit; Serpin E2 ELISA Kit
缩写：SERPINE2
Uniprot No.：Q07235
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 103 | |||||
| Range % | 99-112 | ||||||
| 1:200 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:400 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:800 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of mouse SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2002.1852.3122.2492.1111001.8411.9371.8891.751501.4371.3851.4111.273250.9461.0020.9740.83612.50.5980.5790.5890.4516.250.3620.3740.3680.2303.120.2350.2260.2310.09300.1340.1410.138 | |||||||
货期：3-5 working days
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