首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠色素上皮衍生因子(PEDF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的SERPINF1含量。SERPINF1，也称为色素上皮衍生因子（PEDF），是一种多功能分泌蛋白，具有抗血管生成、抗肿瘤和神经营养功能。其研究机制涉及通过抑制Sost/Sclerostin和其他骨细胞相关基因的表达来调节基质矿化，并通过Erk/GSK-3β/β-连环蛋白信号传导通路调节骨细胞基因表达。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本产品适用于基础科研中探究PEDF在视网膜病变模型中的表达动态、代谢综合征相关机制、肿瘤微环境血管调控以及神经退行性疾病保护作用等研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Serpinf1 ELISA Kit; Pedf ELISA Kit; Sdf3Pigment epithelium-derived factor ELISA Kit; PEDF ELISA Kit; Caspin ELISA Kit; Serpin F1 ELISA Kit; Stromal cell-derived factor 3 ELISA Kit; SDF-3 ELISA Kit
缩写：SERPINF1
Uniprot No.：P97298
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 88-99 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of mouse PEDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-102 | ||||
| EDTA plasma (n=4) | 99 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.3342.2172.2762.1295001.9871.8911.9391.7922501.4361.4141.4251.2781251.0050.9260.9660.81962.50.7180.6980.7080.56131.20.4220.4020.4120.26515.60.2030.2010.2020.05500.1510.1430.147 | ||||||
货期：3-5 working days
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