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产品描述：牛肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPC含量。SFTPC编码的蛋白质是肺表面活性物质的组成部分，对维持肺部功能至关重要。其突变与多种肺部疾病相关，如间质性肺疾病。研究机制主要围绕突变如何影响蛋白结构和功能、干扰表面活性物质稳态，进而引发肺部病变展开。试剂盒检测范围为12.5 ng/mL-800 ng/mL，本产品适用于科研领域探索牛肺部疾病的分子机制、评估肺损伤模型的生物标志物变化，或用于农业研究中监测反刍动物肺部健康状态。为畜牧学、兽医学及基础医学研究提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：SFTPC ELISA Kit; SFTP2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
缩写：SFTPC
Uniprot No.：P15783
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:2 | Average % | 110 | ||||
| Range % | 107-113 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-93 | |||||
回收率：
| The recovery of bovine SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 106 | 101-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.1972.1682.1832.0284001.7051.7321.7191.5642001.1921.1681.1801.0251000.6580.6890.6740.519500.4510.4680.4600.305250.3670.3680.3680.21312.50.2680.2510.2600.10500.1540.1560.155 | ||||||
货期：3-5 working days
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