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Mouse Pulmonary Surfactant-associated protein C (SP-C) ELISA kit

  • 中文名： 小鼠肺表面活性物质相关蛋白C(SP-C) ELISA kit
货号: IPD-E12639m
Price： ￥2500
数量：
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产品详情

  • 产品描述：小鼠肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SFTPC含量。SFTPC编码表面活性蛋白C，是肺表面活性物质的重要成分，对维持肺泡稳定性至关重要。其突变会影响蛋白结构与功能，干扰肺表面活性物质代谢。相关研究聚焦突变致病机制，探索其在间质性肺疾病发病中的作用及潜在治疗靶点。试剂盒检测范围为2.3 ng/mL-150 ng/mL，适用于基础科研中探究肺部疾病模型（如肺纤维化、急性肺损伤）、肺泡上皮细胞功能研究或药物干预下SP - C的表达动态变化。其宽泛的样本兼容性支持体外细胞实验（培养上清）、动物模型（血清/血浆）及组织样本（肺匀浆）的多维度分析，为研究肺部生理病理机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：Sftpc ELISA Kit; Sftp2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SP5 ELISA Kit

  • 缩写：SFTPC

  • Uniprot No.：P21841

  • 种属：Mus musculus (Mouse)

  • 样本类型：serum, plasma, cell culture supernates, tissue homogenates

  • 检测范围：2.3 ng/mL-150 ng/mL

  • 灵敏度：0.58 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Signal Transduction

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %91
    Range %85-96
    1:4Average %95
    Range %90-100
    1:8Average %98
    Range %92-102

  • 回收率：

    The recovery of mouse SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9488-98
    EDTA plasma (n=4)9690-100














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected1502.1472.0522.1002.009751.6931.5761.6351.54437.51.1211.0441.0830.99218.80.6480.6390.6440.5539.40.4290.4240.4270.3364.70.2950.2790.2870.1962.30.1940.1790.1870.09600.0920.0900.091　






  • 货期：3-5 working days

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