首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠性激素结合球蛋白(SHBG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的SHBG含量。SHBG即性激素结合球蛋白，它能特异性结合雄激素和雌激素，调控性激素在体内的活性。近年来研究发现其与多种疾病相关，如多囊卵巢综合征、代谢综合征等。深入研究其作用机制，或可助力相关疾病的早期诊断、病情评估及治疗靶点开发。试剂盒检测范围为1.3 ng/mL-50 ng/mL，适用于研究性激素代谢调控机制、生殖系统疾病模型构建、内分泌干扰物评估等基础科研场景，可满足实验室对小鼠生殖内分泌相关研究的定量检测需求，为探索激素依赖性生理病理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
缩写：SHBG
Uniprot No.：P97497
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：1.3 ng/mL-50 ng/mL
灵敏度：0.33 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of mouse SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 109 | 104-114 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.3442.4912.4182.370271.7681.8221.7951.747131.0031.0421.0230.9753.30.3760.3650.3710.3231.30.1750.1890.1820.13400.0460.0490.048 | ||||||
货期：3-5 working days
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