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产品描述：鸡分泌型免疫球蛋白A(SIgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的SIgA含量。SIgA即分泌型免疫球蛋白 A，是黏膜免疫的关键效应分子。它可阻止病原体附着、中和毒素，在呼吸道、胃肠道等黏膜表面发挥重要保护作用。研究聚焦其产生机制、与疾病关联等，以探寻基于 SIgA 的疾病防治手段。试剂盒检测范围为0.234 μg/mL-60 μg/mL，可满足实验动物模型、疫苗开发等研究中黏膜免疫应答的动态监测需求，例如评估肠道病原感染后的免疫状态、饲料添加剂对黏膜免疫的影响或疫苗免疫效果分析；为禽类免疫学、兽医学及比较医学领域的科研工作者提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SIgA
种属：Gallus gallus (Chicken)
样本类型：serum, plasma
检测范围：0.234 μg/mL-60 μg/mL
灵敏度：0.058 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 86 | ||||
| Range % | 82-89 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 93-101 | |||||
| 1:1600 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 104 | 98-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 600.3450.3460.346 150.5650.5930.579 3.750.8560.8720.864 0.941.1581.1971.178 0.2341.3671.2891.328 01.8811.8251.853 | ||||||
货期：3-5 working days
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