首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
品描述：牛分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测唾液样本中的sIgA含量。sIgA即分泌型免疫球蛋白 A，是黏膜免疫系统的关键效应分子，在抵御病原体入侵方面发挥重要作用。其能阻止病原体黏附于上皮细胞，中和毒素。研究机制着重于其产生调控、与微生物相互作用及在疾病发生发展中的变化。试剂盒检测范围为2.4 μg/mL-150 μg/mL，适用于科研领域中对牛黏膜免疫功能的评估，如病原体感染机制研究、疫苗免疫效果监测、饲料添加剂对黏膜免疫的影响分析等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：sIgA
种属：Bos taurus (Bovine)
样本类型：saliva
检测范围：2.4 μg/mL-150 μg/mL
灵敏度：2.4 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 92 | ||||
| Range % | 85-101 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 92-110 | |||||
回收率：
| The recovery of bovine sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 94-100 | ||||
| EDTA plasma (n=4) | 94 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 1500.2430.2470.245 750.3810.4090.395 37.50.5650.5580.562 18.750.8040.7920.798 9.40.9991.0041.002 4.71.2701.2591.265 2.41.5691.6211.595 02.5152.3362.426 | ||||||
货期：3-5 working days
×
