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产品描述：人硫酸基转移酶1A1(SULT1A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SULT1A1含量。SULT1A1，又称磺基转移酶1A1，是一种参与药物代谢的关键酶，催化多种内源性和外源性化合物的磺化过程，影响其解毒和生物活性。研究显示，SULT1A1在肝癌细胞中具有活性，可能成为开发新型抗癌药物的新靶点。试剂盒检测范围为25 pg/mL-1600 pg/mL，该产品可应用于药物代谢机制研究、环境污染物解毒作用评估、肿瘤等疾病模型中酶活性调控分析，以及特定生理或病理条件下SULT1A1的表达模式探索，为药理学、毒理学及分子生物学领域的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Aryl sulfotransferase 1 ELISA Kit; HAST1/HAST2 ELISA Kit; Human aryl sulfotransferase mRNA complete cds ELISA Kit; MGC131921 ELISA Kit; MGC5163 ELISA Kit; OTTHUMP00000162568 ELISA Kit; OTTHUMP00000162569 ELISA Kit; P PST 1 ELISA Kit; P PST ELISA Kit; P-PST 1 ELISA Kit; Phenol sulfating phenol sulfotransferase 1 ELISA Kit; Phenol sulfotransferase 1 ELISA Kit; Phenol-sulfating phenol sulfotransferase 1 ELISA Kit; PST ELISA Kit; ST1A1 ELISA Kit; ST1A1_HUMAN ELISA Kit; ST1A3 ELISA Kit; STP ELISA Kit; STP1 ELISA Kit; Sulfotransferase 1A1 ELISA Kit; Sulfotransferase family 1A phenol preferring member 1 ELISA Kit; Sulfotransferase family cytosolic 1A phenol preferring member 1 ELISA Kit; Sulfotransferase phenol preferring 1 ELISA Kit; SULT1A1 ELISA Kit; Thermostable phenol sulfotransferase ELISA Kit; Thermostable phenol sulfotransferase1 ELISA Kit; Ts-PST ELISA Kit; TSPST1 ELISA Kit
缩写：SULT1A1
Uniprot No.：P50225
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SULT1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 80-89 | ||||||
| 1:2 | Average % | 103 | |||||
| Range % | 98-107 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human SULT1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-95 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 16002.0782.1482.1131.918 8001.5121.4681.4901.295 4001.0041.0201.0120.817 2000.7330.6890.7110.516 1000.4960.5110.5040.309 500.3810.3740.3780.183 250.2750.2860.2810.086 00.1970.1920.195 | |||||||
货期：3-5 working days
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