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产品描述：小鼠末端补体复合物C5b-9(TCC C5b-9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TCC C5b9含量。小鼠末端补体复合物C5b-9（TCC C5b-9）是补体系统激活终末阶段的产物，通过介导细胞膜穿孔参与免疫防御和炎症反应调控。其在膜性肾病等免疫相关疾病中发挥重要作用，研究其机制有助于揭示疾病发病机制，为疾病治疗提供新靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，该产品适用于科研场景中炎症性疾病模型评估、补体通路功能研究或药物干预效果分析，为探索免疫调节机制提供可靠工具，所有应用仅限于实验室研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TCC C5b9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 84-96 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of mouse TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 97 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.6842.8102.7472.596252.0542.2122.1331.98212.51.4261.4461.4361.2856.250.8540.8670.8610.7103.120.6220.5850.6040.4531.560.3370.3500.3440.1930.780.2580.2640.2610.11000.1480.1540.151 | ||||||
货期：3-5 working days
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