Mouse Serotransferrin(TF) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL023412MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血清转铁蛋白(TF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的TF含量。TF即组织因子，是一种跨膜糖蛋白，作为凝血因子Ⅶ/Ⅶa的细胞膜受体。在生理状态下，TF与血液直接接触机会少。研究机制上，它可启动外源性凝血途径，在止血和血栓形成中起关键作用，也参与多种病理过程如炎症、肿瘤转移等。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，广泛应用于基础研究领域，例如探究铁代谢相关疾病模型、基因修饰小鼠的表型分析、药物干预对铁转运的影响，或作为血液学、营养学及肝脏功能研究的实验工具，为科研人员提供高质量的检测数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Tf ELISA Kit; Trf ELISA Kit; Serotransferrin ELISA Kit; Transferrin ELISA Kit; Beta-1 metal-binding globulin ELISA Kit; Siderophilin ELISA Kit
缩写：
TF
Uniprot No.：
Q921I1
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma
检测范围：
15.6 ng/mL-1000 ng/mL
灵敏度：
3.9 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2000	Average %	96
	Range %	91-103
1:4000	Average %	87
	Range %	83-93
1:8000	Average %	95
	Range %	88-101
1:16000	Average %	93
	Range %	86-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
1000	2.968	2.794	2.881	2.727
500	2.551	2.467	2.509	2.355
250	2.047	2.028	2.038	1.883
125	1.466	1.529	1.498	1.343
62.5	0.916	0.921	0.918	0.764
31.2	0.592	0.613	0.602	0.448
15.6	0.381	0.374	0.378	0.224
0	0.153	0.155	0.154