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产品描述：牛转化生长因子β1(TGF-β1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的TGFB1含量。TGFB1是一种重要的细胞因子，在胚胎发育、组织修复等过程中发挥关键作用。其异常表达与肿瘤发生、纤维化疾病等密切相关。研究机制聚焦于其信号通路，它能调控细胞增殖、分化与凋亡，通过影响下游基因表达参与多种病理生理过程。试剂盒检测范围为6.25 ng/mL-400 ng/mL，该产品适用于兽医科学研究、畜牧养殖健康评估、细胞因子信号通路分析等科研领域，例如探究炎症反应、创伤修复机制或免疫调节实验中TGF-β1的动态变化，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P18341
种属：Bos taurus (Bovine)
样本类型：serum, plasma, cell culture supernates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 83-97 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of bovine TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-97 | ||||
| EDTA plasma (n=4) | 103 | 103-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 4002.6842.6362.6602.537 2002.3482.4542.4012.278 1001.7861.8841.8351.712 501.0310.9971.0140.891 250.5420.5420.5420.419 12.50.3290.3110.3200.197 6.250.2140.2280.2210.098 00.1190.1260.123 | ||||||
货期：3-5 working days
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