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产品描述：鸡转化生长因子β1(TGF-β1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TGFB1含量。TGFB1是重要的靶点，它是一种多功能细胞因子。在细胞生长、分化、凋亡等过程中发挥关键作用。其异常表达与多种疾病如肿瘤、纤维化等密切相关。研究机制主要围绕其信号通路展开，旨在调控其活性来治疗相关疾病。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于家禽免疫学基础研究、病理模型建立及生物标志物分析，特别在探究禽类传染性疾病、疫苗效价评估或应激反应机制等科研场景中，能够为实验提供稳定可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
缩写：TGFB1
Uniprot No.：P09531
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 84-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 84-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 89-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of chicken TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 84-97 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.0112.1052.0581.965101.7901.8281.8091.71651.4661.5271.4971.4042.51.1491.1631.1561.0631.250.7950.8540.8250.7320.6250.4330.4250.4290.3360.3120.2640.2550.2600.16700.0950.0910.093 | ||||||
货期：3-5 working days
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