首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠转化生长因子β受体3(TGFBR3)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TGFBR3含量。TGFBR3 是重要的转化生长因子-β 超家族辅助受体。其与肿瘤、心血管等疾病的发生发展密切相关。研究机制上，它参与细胞生长、分化和迁移等多种信号通路的调控，通过影响细胞生物学行为发挥作用，是药物研发和疾病治疗的潜在靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于细胞信号转导研究、疾病模型构建及分子机制探索等科研场景，为探索 TGF-β信号通路调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tgfbr3 ELISA Kit; Transforming growth factor beta receptor type 3 ELISA Kit; TGF-beta receptor type 3 ELISA Kit; TGFR-3 ELISA Kit; Betaglycan ELISA Kit; Transforming growth factor beta receptor III ELISA Kit; TGF-beta receptor type III ELISA Kit
缩写：TGFBR3
Uniprot No.：O88393
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGFBR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 96-107 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 86-97 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 93-102 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 81-92 | ||||||
回收率：
| The recovery of mouse TGFBR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 89 | 84-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected4002.7542.8742.8142.6022002.3322.4442.3882.1761001.8231.7781.8011.589501.1561.1341.1450.933250.6240.6050.6150.40312.50.4230.4110.4170.2056.250.3180.3140.3160.10400.2120.2110.212 | |||||||
货期：3-5 working days
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