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Human cardiac isoform of Tropnin T,cTnT ELISA Kit

  • 中文名： 人心肌特异性肌钙蛋白T(cTnT)ELISA Kit
货号: IPD-E09340h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人心肌特异性肌钙蛋白T(cTnT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TNNT2含量。TNNT2是重要的心肌肌钙蛋白T亚基，在心肌收缩与舒张中起关键作用。其异常表达与多种心血管疾病密切相关，如心肌梗死等。研究多围绕检测其血中水平用于疾病诊断、探究其结构功能变化影响心肌力学特性的机制等。试剂盒检测范围为15.6 pg/ml-1000 pg/ml，适用于科研领域中心肌损伤相关机制研究，适用于心血管疾病动物模型评估、体外心肌细胞损伤实验分析，或药物干预后心肌保护效应的动态监测等科研场景，为探索心肌损伤病理机制及潜在治疗策略提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cardiac muscle troponin T ELISA Kit; Cardiomyopathy dilated 1D (autosomal dominant) ELISA Kit; Cardiomyopathy hypertrophic 2 ELISA Kit; CMD1D ELISA Kit; CMH2 ELISA Kit; CMPD2 ELISA Kit; cTnT ELISA Kit; LVNC6 ELISA Kit; MGC3889 ELISA Kit; OTTHUMP00000033864 ELISA Kit; OTTHUMP00000033865 ELISA Kit; OTTHUMP00000033866 ELISA Kit; OTTHUMP00000033867 ELISA Kit; OTTHUMP00000033870 ELISA Kit; OTTHUMP00000218095 ELISA Kit; RCM3 ELISA Kit; TNNT 2 ELISA Kit; TNNT2 ELISA Kit; TNNT2_HUMAN ELISA Kit; TnTc ELISA Kit; Troponin T cardiac muscle ELISA Kit; Troponin T type 2 (cardiac) ELISA Kit; Troponin T type 2 cardiac ELISA Kit; Troponin T, cardiac muscle ELISA Kit; Troponin T2 cardiac ELISA Kit
  • 缩写：
    TNNT2
  • Uniprot No.：
    P45379
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml-1000 pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human cTnT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    101


    Range %

    95-108


    1:2

    Average %

    97


    Range %

    92-101


    1:4

    Average %

    86


    Range %

    82-90


    1:8

    Average %

    93


    Range %

    88-97


  • 回收率：

    The recovery of human cTnT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-98


    EDTA plasma (n=4)

    95

    90-99


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.113

    2.147

    2.130

    1.954


    500

    1.661

    1.715

    1.688

    1.512


    250

    1.318

    1.386

    1.352

    1.176


    125

    1.072

    1.054

    1.063

    0.887


    62.5

    0.753

    0.732

    0.743

    0.567


    31.2

    0.604

    0.581

    0.593

    0.417


    15.6

    0.325

    0.337

    0.331

    0.155


    0

    0.179

    0.173

    0.176




  • 货期：
    3-5 working days

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