| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCT |
| Uniprot No | P06132 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MEANGLGPQG FPELKNDTFL RAAWGEETDY TPVWCMRQAG RYLPEFRETR AAQDFFSTCR SPEACCELTL QPLRRFPLDA AIIFSDILVV PQALGMEVTM VPGKGPSFPE PLREEQDLER LRDPEVVASE LGYVFQAITL TRQRLAGRVP LIGFAGAPWT LMTYMVEGGG SSTMAQAKRW LYQRPQASHQ LLRILTDALV PYLVGQVVAG AQALQLFESH AGHLGPQLFN KFALPYIRDV AKQVKARLRE AGLAPVPMII FAKDGHFALE ELAQAGYEVV GLDWTVAPKK ARECVGKTVT LQGNLDPCAL YASEEEIGQL VKQMLDDFGP HRYIANLGHG LYPDMDPEHV GAFVDAVHKH SRLLRQN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCT(降钙素原)重组蛋白研究的示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
---
1. **标题**: *"High-level expression and purification of recombinant human procalcitonin in Escherichia coli"*
**作者**: Zhang L, et al.
**摘要**: 研究报道了利用大肠杆菌表达系统高效表达重组人降钙素原(PCT)的方法，通过优化诱导条件和亲和层析技术获得高纯度蛋白，为临床检测试剂开发提供基础。
2. **标题**: *"Functional characterization of recombinant procalcitonin as a sepsis biomarker in vitro"*
**作者**: Müller B, et al.
**摘要**: 分析了重组表达的PCT蛋白在体外炎症模型中的生物学活性，证实其与天然PCT在激活免疫信号通路中的等效性，支持其在败血症诊断中的应用价值。
3. **标题**: *"Development of a novel monoclonal antibody against recombinant PCT for point-of-care testing"*
**作者**: Chen Y, et al.
**摘要**: 利用重组PCT蛋白作为抗原，成功筛选出高特异性单克隆抗体，并基于此开发了快速检测试纸条，提升了床旁检测的灵敏度和便捷性。
---
**建议**：实际文献请通过PubMed、Web of Science等平台搜索关键词(如“recombinant procalcitonin expression”、“PCT biomarker production”)获取最新研究。
Procalcitonin (PCT), a 116-amino-acid precursor of calcitonin, is a protein encoded by the *CALC-1* gene. Under normal physiological conditions, PCT is primarily produced by thyroid C-cells and rapidly cleaved into calcitonin, a hormone involved in calcium homeostasis. However, during systemic inflammation—particularly bacterial infections—PCT expression is upregulated in extra-thyroid tissues (e.g., liver, adipose, and immune cells) via cytokine-mediated pathways. This elevated PCT circulates intact, making it a clinically valuable biomarker for sepsis, severe bacterial infections, and inflammatory conditions.
Recombinant PCT refers to the protein produced through genetic engineering, bypassing natural cellular pathways. It is synthesized in heterologous systems like *E. coli* or mammalian cell cultures, ensuring high purity and consistency for research and diagnostic applications. Recombinant PCT retains immunoreactivity, enabling its use as a reference standard in PCT detection assays (e.g., ELISA, lateral flow tests) to quantify endogenous PCT levels in patient samples.
Therapeutic exploration of recombinant PCT remains limited, but its role in modulating inflammatory responses is under investigation. Studies suggest PCT may interact with immune receptors (e.g., TLRs) or adhesion molecules, influencing cytokine release and endothelial activation. Challenges include optimizing stability and minimizing aggregation during production. Current applications focus on improving sepsis diagnostics, antibiotic stewardship programs, and understanding PCT's pathophysiological mechanisms. Future directions may explore its potential as a therapeutic target or adjunct in infection management.
×
