关键信息
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基因名
SLC25A4
- 应用
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别名
ADP,ATP carrier protein 1; ADP,ATP carrier protein, heart/skeletal muscle isoform T1; Adenine nucleotide translocator 1; Solute carrier family 25 member 4
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种属
Human
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表达系统
E. coli Cell-free
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标签
N-10*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P12235
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表达区间
G2-V298
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氨基酸序列
GDHAWSFLKDFLAGGVAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV
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蛋白长度
Full Length of Mature Protein
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分子量
30-38 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SLC25A4, also known as the adenine nucleotide translocator 1 (ANT1), is a pivotal protein encoded by the SLC25A4 gene, primarily located in the inner mitochondrial membrane. It plays a critical role in the transport of adenine nucleotides across the mitochondrial membrane, facilitating ATP/ADP exchange essential for cellular energy metabolism. Mutations or dysfunctions in SLC25A4 have been implicated in various mitochondrial disorders, leading to significant clinical manifestations such as exercise intolerance, cardiomyopathy, and neurodegeneration. Given its fundamental role in cellular energy homeostasis, SLC25A4 is a focal point for research aimed at understanding mitochondrial pathologies and developing potential therapeutic strategies. The study of recombinant SLC25A4 protein enables detailed characterization of its transport mechanisms, interaction with other mitochondrial proteins, and the impact of specific mutations on its function. By utilizing recombinant protein technology, researchers can produce large quantities of SLC25A4 for biochemical assays, structural analysis, and drug screening, enhancing our understanding of its physiological roles and the pathophysiological consequences of its dysfunction. Furthermore, insights gained from SLC25A4 research may pave the way for novel interventions targeting mitochondrial-related diseases, highlighting the necessity of continued exploration into the complex dynamics of this important transporter protein.
