首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血栓素B2(TXB2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TXB2含量。TXB2是一种由TXA2酶促代谢产生的稳定水解产物，是TXA2的稳定代谢物，可以反映TXA2的全身生物合成速率。TXB2在生理和病理过程中扮演重要角色，如参与止血、动脉粥样硬化、血栓形成等。研究TXB2有助于了解相关疾病的发生机制，为疾病诊断和治疗提供新的靶点。试剂盒检测范围为3.9 pg/mL-250 pg/mL，适用于心血管疾病动物模型研究、抗血小板药物药效评估、炎症相关机制探索等科研领域，满足基础研究中对TXB2动态变化的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TXB2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 pg/mL-250 pg/mL
灵敏度：0.97 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TXB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-106 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of rat TXB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-103 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2501.9901.8901.9401.7851251.2981.1981.2481.09362.50.7860.7660.7760.62131.250.5450.5250.5350.38015.60.3410.3210.3310.1767.80.2920.2720.2820.1273.90.2150.2110.2130.05800.1560.1540.155 | ||||||
货期：3-5 working days
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