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产品描述：人P53抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的P53 Ab含量。P53是重要的抑癌基因，P53 Ab即P53抗体。其背景在于P53功能异常与多种肿瘤发生发展密切相关。研究机制方面，聚焦于P53 Ab与P53蛋白结合，以此监测P53状态，对于研究肿瘤发生机制、判断预后及开发治疗策略有重要意义。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研场景中定量分析P53在肿瘤机制研究、药物作用评估或细胞应激反应中的表达水平变化，为癌症生物学、分子病理学等领域的研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：P53 Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human P53 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 85-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 84-99 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 82-105 | |||||
回收率：
| The recovery of human P53 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-96 | ||||
| EDTA plasma (n=4) | 94 | 87-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.6892.6622.6762.570 502.1312.1392.1352.029 251.4671.4791.4731.367 12.50.8420.8840.8630.757 6.250.4790.4870.4830.377 3.120.3160.3040.3100.204 1.560.2470.2540.2510.145 00.1070.1050.106 | ||||||
货期：3-5 working days
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