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Human C-C motif chemokine 26(CCL26) ELISA kit

  • 中文名： 人趋化因子26(CCL26) ELISA kit
货号: IPD-EL004790HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人趋化因子26(CCL26)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL26含量。CCL26，又称Eotaxin-3，是一种CC趋化因子，主要在心脏、肺和卵巢等组织中表达，对嗜酸性粒细胞和嗜碱性粒细胞具有趋化作用，参与过敏和其他疾病的发病机制，如嗜酸性粒细胞性食管炎和Churg-Strauss综合征。CCL26通过结合CCR3或CX3CR1受体，诱导嗜酸性粒细胞迁移，在炎症反应中发挥重要作用。试剂盒检测范围为39 pg/ml- 2500 pg/ml，灵敏度为9.75 pg/ml。广泛应用于基础研究领域，包括炎症性疾病模型中的细胞因子网络分析、药物干预实验的疗效评估以及免疫微环境调控机制的探索，为呼吸道疾病、皮肤炎症及免疫代谢相关研究提供高效检测工具，所有检测结果仅用于科研用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C-C motif chemokine 26 ELISA Kit; CC chemokine IMAC ELISA Kit; CCL 26 ELISA Kit; CCL26 ELISA Kit; CCL26_HUMAN ELISA Kit; Chemokine (C C motif) ligand 26 ELISA Kit; chemokine N1 ELISA Kit; Eotaxin 3 ELISA Kit; Eotaxin-3 ELISA Kit; IMAC ELISA Kit; Macrophage inflammatory protein 4-alpha ELISA Kit; MIP 4a ELISA Kit; MIP 4alpha ELISA Kit; MIP-4-alpha ELISA Kit; MIP4a ELISA Kit; MIP4alpha ELISA Kit; SCYA 26 ELISA Kit; SCYA26 ELISA Kit; Small inducible cytokine A26 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 26 ELISA Kit; Small inducible cytokine subfamily A member 26 ELISA Kit; Small-inducible cytokine A26 ELISA Kit; Thymic stroma chemokine 1 ELISA Kit; Thymic stroma chemokine-1 ELISA Kit; TSC 1 ELISA Kit; TSC-1 ELISA Kit; TSC1 ELISA Kit
  • 缩写：
    CCL26
  • Uniprot No.：
    Q9Y258
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    39 pg/ml - 2500 pg/ml
  • 灵敏度：
    9.75 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human CCL26 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    91


    Range %

    86-95


    1:2

    Average %

    102


    Range %

    97-107


    1:4

    Average %

    91


    Range %

    85-97


    1:8

    Average %

    96


    Range %

    91-103


  • 回收率：

    The recovery of human CCL26 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    95

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2500

    2.144

    2.168

    2.156

    2.041


    1250

    1.618

    1.600

    1.609

    1.494


    625

    1.203

    1.256

    1.230

    1.115


    312.5

    0.847

    0.800

    0.824

    0.709


    156.25

    0.635

    0.601

    0.618

    0.503


    78

    0.312

    0.332

    0.322

    0.207


    39

    0.236

    0.241

    0.239

    0.124


    0

    0.114

    0.116

    0.115




  • 货期：
    3-5 working days

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