首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠嗜酸粒细胞趋化蛋白Eotaxin 3(Eotaxin 3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Eotaxin 3含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于研究炎症性疾病模型中嗜酸性粒细胞募集机制、评估实验性抗过敏药物对趋化因子表达的调控作用，或解析免疫细胞与微环境相互作用的分子基础，为呼吸道疾病、皮肤过敏等病理机制研究提供可靠工具。试剂盒操作流程标准化，适用于体外实验环境下多种生物样本的平行检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：Eotaxin 3
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Eotaxin 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 90-93 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 101-105 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-94 | |||||
回收率：
| The recovery of mouse Eotaxin 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 90-94 | ||||
| EDTA plasma (n=4) | 89 | 85-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.9272.8742.9012.7452002.0152.1352.0751.9191001.3031.4031.3531.197500.8010.8220.8120.656250.5320.5240.5280.37212.50.3190.3320.3260.1706.250.2440.2540.2490.09300.1510.1600.156 | ||||||
货期：3-5 working days
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