| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFAND5 |
| Uniprot No | O76080 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMCRKKVGLTGFD CRCGNLFCGL HRYSDKHNCP YDYKAEAAAK IRKENPVVVA EKIQRI |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZFAND5重组蛋白的3篇参考文献的示例(注：内容为虚构，仅供参考)：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant ZFAND5 in Ubiquitin-Proteasome Regulation*
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组ZFAND5蛋白，并解析其晶体结构。功能实验表明，ZFAND5通过AN1锌指结构域与泛素连接酶相互作用，增强底物泛素化效率，提示其在蛋白酶体降解途径中的调控作用。
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2. **文献名称**：*ZFAND5 Facilitates Stress-Induced Protein Aggregation Clearance via Recombinant Chaperone Activity Assays*
**作者**：Smith JR, Tanaka K, et al.
**摘要**：利用重组ZFAND5蛋白进行体外分子伴侣功能分析，发现其在热应激条件下与错误折叠蛋白结合，并通过招募HSP70和泛素化系统促进聚集蛋白的清除，为ZFAND5在细胞应激响应中的机制提供依据。
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3. **文献名称**：*Role of Recombinant ZFAND5 in Modulating Autophagy-Lysosomal Pathway in Neurodegenerative Disease Models*
**作者**：Gomez M, Lee S, et al.
**摘要**：通过体外表达的重组ZFAND5蛋白，研究证实其通过调控mTOR信号通路增强自噬活性，减少tau蛋白和α-突触核蛋白的异常聚集，为神经退行性疾病的治疗提供潜在靶点。
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以上示例结合了ZFAND5在蛋白质降解、结构分析和疾病模型中的可能研究方向，实际文献需通过学术数据库(如PubMed、Web of Science)检索。
ZFAND5 (Zinc Finger AN1-Type Domain 5) is a protein-coding gene that belongs to the AN1-type zinc finger protein family, characterized by conserved cysteine-rich domains involved in protein-protein interactions and stress response regulation. The encoded ZFAND5 protein contains an AN1-type zinc finger domain and a ubiquitin-like fold, which are critical for its roles in cellular processes such as ubiquitination, proteasomal degradation, and cellular adaptation to stress. Studies suggest ZFAND5 functions as a regulatory component of the ubiquitin-proteasome system (UPS), potentially acting as a scaffold or adaptor to facilitate substrate recognition or modulate proteasome activity under stress conditions like oxidative or endoplasmic reticulum stress.
Recombinant ZFAND5 protein is produced using expression systems (e.g., E. coli, mammalian cells) to enable functional and structural studies. Its recombinant form allows researchers to investigate interactions with proteasome subunits, ubiquitin ligases, or stress-response pathways. Emerging evidence links ZFAND5 dysregulation to diseases, including cancer and neurodegenerative disorders, where impaired protein homeostasis is a hallmark. For example, ZFAND5 has been implicated in tumor progression via modulating NF-κB signaling or apoptosis, while its role in clearing misfolded proteins highlights therapeutic potential for conditions like Alzheimer’s disease.
Research on recombinant ZFAND5 also explores its post-translational modifications, subcellular localization, and tissue-specific expression patterns. Despite progress, its exact molecular mechanisms remain under investigation, particularly regarding how its zinc finger domains mediate specific interactions. Current studies focus on leveraging recombinant ZFAND5 to develop targeted therapies or biomarkers for diseases linked to proteasomal dysfunction or oxidative stress.
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