| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZCCHC12 |
| Uniprot No | Q6PEW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-402aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMASIIAR VGNSRRLNAP LPPWAHSMLR SLGRSLGPIM ASMADRNMKL FSGRVVPAQG EETFENWLTQ VNGVLPDWNM SEEEKLKRLM KTLRGPAREV MRVLQATNPN LSVADFLRAM KLVFGESESS VTAHGKFFNT LQAQGEKASL YVIRLEVQLQ NAIQAGIIAE KDANRTRLQQ LLLGGELSRD LRLRLKDFLR MYANEQERLP NFLELIRMVR EEEDWDDAFI KRKRPKRSES MVERAVSPVA FQGSPPIVIG SADCNVIEID DTLDDSDEDV ILVESQDPPL PSWGAPPLRD RARPQDEVLV IDSPHNSRAQ FPSTSGGSGY KNNGPGEMRR ARKRKHTIRC SYCGEEGHSK ETCDNESDKA QVFENLIITL QELTHTEMER SRVAPGEYND FSEPL |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZCCHC12重组蛋白的示例参考文献(注：部分为假设性描述，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Recombinant expression and functional characterization of ZCCHC12 as a RNA-binding protein in neuronal development"*
**作者**: Liu X, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化带His标签的ZCCHC12重组蛋白，证实其在体外结合特定RNA序列的能力，并发现其通过调控mRNA稳定性参与神经突触形成。
2. **文献名称**: *"Structural insights into the zinc finger domain of ZCCHC12 and its role in protein-RNA interaction"*
**作者**: Wang Y, et al.
**摘要**: 利用哺乳动物细胞表达系统获得高纯度ZCCHC12重组蛋白，结合X射线晶体学解析其锌指结构域的三维结构，揭示了该区域对RNA结合的分子机制及突变导致的结合能力丧失。
3. **文献名称**: *"ZCCHC12 recombinant protein suppresses tumor growth by modulating miRNA processing"*
**作者**: Chen L, et al.
**摘要**: 通过昆虫杆状病毒系统表达ZCCHC12重组蛋白，发现其通过调控Drosha复合体的活性影响miRNA成熟，在体外实验中显著抑制癌细胞增殖。
4. **文献名称**: *"Development of a ZCCHC12 recombinant protein-based assay for Alzheimer's disease biomarker screening"*
**作者**: Kim J, et al.
**摘要**: 研究构建了基于HEK293细胞表达的ZCCHC12重组蛋白的ELISA检测方法，验证其与脑脊液中Aβ寡聚体的特异性结合，提出其在阿尔茨海默病早期诊断中的应用潜力。
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**注意**：以上文献为示例，实际引用时请通过PubMed或Google Scholar检索真实文献，并核对研究内容相关性。若研究较少，可扩展至ZCCHC家族其他成员或相关功能通路。
ZCCHC12 is a member of the zinc finger CCHC-type (ZCCHC) protein family, characterized by conserved cysteine-histidine motifs involved in nucleic acid binding or protein interactions. This recombinant protein, generated via genetic engineering, is typically expressed in vitro to study its biological roles. ZCCHC12 has garnered interest due to its potential involvement in neurodevelopment and diseases such as Alzheimer's and cancer. Structurally, it contains a CCHC-type zinc finger domain, suggesting RNA-binding properties that may regulate post-transcriptional processes like RNA stability or translation.
Research indicates ZCCHC12 interacts with critical signaling pathways, including Wnt and Notch, which are essential for cellular differentiation and oncogenesis. Its dysregulation has been observed in certain cancers, with studies reporting upregulated expression in tumor tissues linked to poor prognosis. Additionally, ZCCHC12 may influence epigenetic regulation by modulating non-coding RNAs or chromatin modifiers, though mechanistic details remain unclear.
Recombinant ZCCHC12 proteins, often tagged with His or GST for purification, enable functional assays such as protein-RNA interaction studies, enzymatic activity analyses, and antibody production. These tools help elucidate its role in disease pathways and validate its potential as a therapeutic target. Despite progress, the protein's full interactome and regulatory networks require further exploration. Current investigations focus on linking its molecular functions to pathological conditions, aiming to develop diagnostic or therapeutic strategies targeting ZCCHC12-associated mechanisms.
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